Flouvista BIOsupply

FluoVista - endpoint Fluorescence measurement for HLA-, RBC- and HPA-diagnostics

FluoGene is a unique method for molecular HLA-, RBCand HPA typing combining all advantages of the SSPPCR with the speed of endpoint fluorescence detection. The analysis is based on a specifically modified TaqMan probe system by inno-train. Up to four fluorophores are detected by the FluoVista analyzer and an integrated evaluation is realized by the FluoGene software. Plate-specific data can be easily read from the barcoded FluoGene PCR plate and sample-ID can be scanned and automatically transferred into the FluoGene Software. A fully automated workflow can be realized by the use of our small pipetting unit PiU1. Just scan the barcode of the FluoGene PCR plate for an automated protocol selection („Scan & Go“). FluoGene is a self-contained system comprising the ready-to-use kits, the detection device and the software solution displaying the typing results immediately after the evaluation read. For the PCR set-up simply add your diluted sample DNA to the ready-to-use FluoMix and dispense 15 μl per well over the PCR plate. This manual step can be performed automated by the pipetting unit PiU1. Afterwards the FluoGene plates are sealed with an adhesive optical foil. The subsequent pre- and postreads are generated in a closed environment. Therefore any risk of a post-PCR contamination gets eliminated. The FluoGene Software supplies the immediate display of typing results, as well as a fully-automated data management.

FluoQuant - fluorescence based DNA quantification with the FluoVista Analyzer

DNA concentration can be rapidly measured in 96 well format using fluorescent dyes. This enables a very fast, accurate, reasonable priced and automated process - measurement and typing of your samples in one system! The system is validated with QuantiFluor® by Promega, a fluorescence dye intercalating specifically in double-stranded DNA for specific and sensitive quantification. Just add your sample and dye to a 96 well plate - that`s all.